The ED50 was found to be 1ng/ml corresponding to a Specific Activity of 1MIU/mg calculated by the ability to stimulate the proliferation of mouse 3T3 fibroblasts (PNAS 94, 10205, 1997. Biochemistry, 1996, 35, 12077).
The protein was lyophilized with 20mM sodium phosphate buffer.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98.0% as determined by SDS-PAGE.
It is recommended to reconstitute the lyophilized PDGF-AA in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized PDGF-AA although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution PDGF-AA should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.