Proliferating Cell Antigen Human Recombinant, Sf9
PCNA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 29,794 Dalton.
PCNA is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Accession
Applications
Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Coating concentration
0.5-1.1 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Formulation
Recombinant Human PCNA is supplied in16mM HEPES buffer pH-8, 400mM NaCl, and 20% glycerol.
Immunological Function
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera.
Physical Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Source
Storage
Store at 4oC if entire vial will be used within 2-4 weeks.
Store, frozen at -20oC for longer periods of time.
Avoid multiple freeze-thaw cycles.
Synonyms
Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367.
