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Recombinant 2-oxoglutarate dehydrogenase E1 component (odhA)

Recombinant 2-oxoglutarate dehydrogenase E1 component (odhA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP858196SKYYeast1mgQuote
EP858196SKYE. coli1mgQuote
BP858196SKYBaculovirus200ugQuote
MP858196SKYMammalian Cell200ugQuote

Protein Information

SpeciesStaphylococcus aureus (strain N315)
UniProt IDQ99U74
Gene NameodhA; Locus:SA1245
Protein Name2-oxoglutarate dehydrogenase E1 component
Region Expressed1-910
Expression Tag6xHis
Purity>90%
AA SequenceMTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLFSTIKRVMRLIDNIRQY GHLKADIYPVNPPKRKHVPKLEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRME KRYKGPIAFEYTHINNNTERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKN FVGAKRFSIEGVDALVPMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMIS EFMHTDPMKFLPEDGSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAP VVEGRTRAAQDDTQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGS LHIITNNRIGFTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEF HKDVVIDLVGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHS FIEQVQKELRQAHDKINKADKMDNPDMEKPAELALPLQADEQSFTFDHLKEINDALLTYP DGFNILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFS HRHAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAAEN NCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSGGFEP ILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIEALLAQL PKLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDGEIHKLVQN KIIENALKNN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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