Request QuoteCatalog Number: xP510228GFJSize: 0.2-1mg

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Recombinant 2-oxoglutarate dehydrogenase E1 component (odhA)

Recombinant 2-oxoglutarate dehydrogenase E1 component (odhA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP510228GFJYeast1mgQuote
EP510228GFJE. coli1mgQuote
BP510228GFJBaculovirus200ugQuote
MP510228GFJMammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus sp. (strain WCH70)
UniProt IDC5D802
Gene NameodhA; Locus:GWCH70_0919
Protein Name2-oxoglutarate dehydrogenase E1 component
Region Expressed1-952
Expression Tag6xHis
Purity>90%
AA SequenceMTKQTMNYAEPWSQFYGPNLGYVMEMYEQYLEDPDSVDPELKQLFKEWGAPTTEAERFDH SESAAKTYQTFRLPENPTIFSKLVAAVKLADKIRHYGHLAADINPLNTQNKDTRRIELSE FDLTEDDLKQIPVAFICPHAPAHVKNGLDAINHLRKIYTDKIAFEFSQVHNLEERNWLIS QIESGAYYPSLTNEEKVALLRRLTEVEGFEKFLHRTFVGQKRFSIEGLDSMVPLLDELIR HSIEEEVKAVNIGMAHRGRLNVLAHVLGKPYEMIFAEFQHAESKDFMPSEGSVAITYGWT GDVKYHLGAARRLRNKNEHTMRITLANNPSHLEVVNPVVLGFTRAAQEDRSNAGVPSQDT DSAFAIMIHGDAAFPGQGIVAETLNLSRLQGYQTGGSIHIIANNMIGFTTESYDSRSTKY ASDIAKGFEIPIVHVNADDPEACLAAANLAFAYRKRFKKDFVIDLIGYRRFGHNEMDEPM ATNPTMYSIIQQHPTVRQLYAQKLIEKGIITKEAVEEMEREVAERLKIAYEKVPKDESKL DFIMDPPKPVASKLPFVKTSVEKDVLRRLNKELLQFPSDFHVFNKLERILKRREGVFDGK GKIDWAHAEILAFATILRDGVPIRLTGQDSQRGTFAQRHLVLHDMKTGEEFVPLHHISDA NASFVVYNSPLTEAAVLGYEYGYNVFAPETLVLWEAQFGDFANMAQVMFDQFISSGRAKW GQKSGLVMLLPHGYEGQGPEHSSGRLERFLQLAAENNWTVANLSTAAQYFHILRRQAGIL QREEVRPLVLMTPKSLLRHPLAASDVEEFTNGQFHPVIEQKGLGENREKVERIILCTGKF AIDLAEQINKMEGLDWLHIVRVEELYPFPKEELQAIFARYPNVKEIIWAQEEPKNMGSWC YVEPKLREIAPDEVDVSYIGRRRRASPAEGDPVVHRKEQERIIQCALTKKEQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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