Request QuoteCatalog Number: xP514116HXSSize: 0.2-1mg

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Recombinant 2-oxoglutarate carboxylase large subunit (cfiA)

Recombinant 2-oxoglutarate carboxylase large subunit (cfiA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP514116HXSYeast1mgQuote
EP514116HXSE. coli1mgQuote
BP514116HXSBaculovirus200ugQuote
MP514116HXSMammalian Cell200ugQuote

Protein Information

SpeciesHydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6)
UniProt IDD3DJ41
Gene NamecfiA; Locus:HTH_1392, Hydth_1382
Protein Name2-oxoglutarate carboxylase large subunit
Region Expressed1-652
Expression Tag6xHis
Purity>90%
AA SequenceMQAVEIMEEIREKFKEFEKGGFRKKILITDLTPRDGQQCKLATRVRTDDLLPLCEAMDKV GFYAVEVWGGATYDVCLRYLKEDPWERLRRIKEVMPNTKLQMLFRGQNIVGYRPKSDKLV YKFVERAIKNGITVFRVFDALNDNRNIKTAVKAIKELGGEAHAEISYTRSPIHTYQKWIE YALEIAEMGADWLSFKDATGIIMPFETYAIIKGIKEATGGKLPVLLHNHDMSGTAIVNHM MAVLAGVDMLDTVLSPLAFGSSHPATESVVAMLEGTPFDTGIDMKKLDELAEIVKQIRKK YKKYETEYAGVNAKVLIHKIPGGMISNMVAQLIEANALDKIEEALEEVPNVERDLGHPPL LTPSSQIVGVQAVLNVISGERYKVITKEVRDYVEGKYGKPPGPISKELAEKILGPGKEPD FSIRAADLADPNDWDKAYEETKAILGREPTDEEVLLYALFPMQAKDFFVAREKGELHPEP VDELVETTEVKAGVVPGAAPVEFEIVYHGEKFKVKVEGVSAHQEPGKPRKYYIRVDGRLE EVQITPHVEAIPKGGPTPTAVQAEEKGIPKATQPGDATAPMPGRVVRVLVKEGDKVKEGQ TVAIVEAMKMENEIHAPISGVVEKVFVKPGDNVTPDDALLRIKHIEEEVSYG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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