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Recombinant 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (ubiF)

Recombinant 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (ubiF) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP301152ENVYeast1mgQuote
EP301152ENVE. coli1mgQuote
BP301152ENVBaculovirus200ugQuote
MP301152ENVMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain K12)
UniProt IDP75728
Gene NameubiF; aka: yleB; Locus:b0662, JW0659
Protein Name2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase
Region Expressed1-391
Expression Tag6xHis
Purity>90%
AA SequenceMTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVADSQPDVRISAISAASV SLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKLPLLGYMVENTVLQQALWQ ALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKLVIGADGANSQVRQMAGIGVH AWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPLFDNWASLVWYDSPARIRQLQNM NMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQG VNLGYRDVDALIDVLVNARSYGEAWASYPVLKRYQMRRMADNFIMQSGMDLFYAGFSNNL PPLRFMRNLGLMAAERAGVLKRQALKYALGL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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