The specific activity was determined by the dose dependent inhibition of rhBMP4 induced alkaline phosphate production by ATDC5 cells and is typically 20-100ng/ml.
The Noggin was lyophilized from 1mg/ml in 1xPBS.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95% as obsereved by SDS-PAGE.
It is recommended to reconstitute the lyophilized Noggin in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Noggin although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Noggin should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
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