NANOG-TAT Human Recombinant
NANOG Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 305 amino acids and having a molecular mass of 34.6kDa.
The NANOG is fused to an N-terminal TAT (transcriptional activator protein) tag and purified by proprietary chromatographic techniques.
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gly-Arg-Lys-Lys.
Formulation
NANOG was lyophilized after extensive dialysis against PBS.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized NANOG in sterile PBS not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Stability
Lyophilized NANOG although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution NANOG should be stored at 4oC between 2-7 days and for future use below -18oC.
Please prevent freeze-thaw cycles.
Synonyms
NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.
Accession
