Nanog Human Recombinant, His Tag
NANOG Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 174 amino acids (1-154) and having a molecular mass of 19.6 kDa.
The NANOG is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQ
Formulation
NANOG protein (0.25mg/ml) is supplied in 20mM Tris-HCL, pH-8, 1mM DTT and 20% Glycerol.
Physical Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Source
Stability
Store at 4oC if entire vial will be used within 2-4 weeks. Store, frozen at -20oC for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Synonyms
NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.
Accession
