$90.00Catalog Number: MAB422-5ugSize: 5ug

NANOG Antibody

Homeobox Protein NANOG, Mouse Anti Human
Monoclonal Antibody

Monoclonal antibodies provide the best target specificity and affinity. It is also advantagous for long term projects as there is little lot to lot variation, where polycolonal antibodies will vary significantly with different preparations due to immunogen preparation and animal responses.

Applications

NANOG antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500~1:1,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:500 and Immunohistochemistry is 1:50.

Clone

P5A10AT.

Ig Subclass

Mouse IgG2a heavy chain and κ light chain.

Immunogen

Anti-human NANOG mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human NANOG amino acids 1-154 purified from E. coli.

Protein formulation

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

Purification Method

NANOG antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Stability / Shelf Life

12 months at -20oC. 1 month at 4oC.

Storage Procedures

For periods up to 1 month store at 4oC, for longer periods of time, store at -20oC. Prevent freeze thaw cycles.

Synonyms

NANOG, Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog.

Type

Mouse Anti Human Monoclonal.

Quantity

$90.00

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