Recombinant Mouse Interleukin-6 (rmIL-6)
Interleukin-6 (IL-6) is a pro-inflammatory cytokine that also has an important role in immunity. IL-6 induces growth and terminal differentiation of B cells; secretion of immunoglobulins; differentiation and activation of T cells and macrophages; and the induction of acute-phase response proteins. Many types of cells, including macrophages, T cells, fibroblasts, and endothelial cells, produce IL6 in response to stimuli such as bacteria, viruses, and other cytokines, particularly IL-1 and tumor necrosis factor, alpha (TNF). Human IL-6 is active on both mouse and rat cells, while mouse IL-6 has no activity on human cells. Recombinant mouse IL6 is a 22 kDa protein containing 189 amino acid residues.
Other Sizes
| BX1006-20: | 20 ug |
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| BX1006-200: | 200 ug |
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| BX1006-1000: | 1 mg |
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Custom size is also available upon request.
Specifications
| Lot Number: | On label |
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| Formulation: | Lyophilized powder lyophilized from a volatile buffer (50 mM NH4HCO3 , pH 8.0). |
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| Preservative: | None |
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| MW: | 22kD |
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| Purity: | >97% on 15% SDS-PAGE |
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| Source: | Recombinant mature protein expressed in E. coli (189 amino acid residues). |
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| Sterility: | 0.2 um membrane-filtered and packaged aseptically. |
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| ED50: | ND |
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| Endotoxin: | ≤0.1 EU/ug protein |
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| QC test: | SDS-PAGE |
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Note: Endotoxin Unit (EU) is determined by Limulus Amebocyte Lysate (LAL) assay.
Reconstitution and Use
Reconstitute the contents of the vial using sterile phosphate-buffered saline (PBS) to a concentration no less than 100 ug/ml and aliquot for future use. (If the initial rehydration is too dilute, activity may be lost due to the non-specific adsorption to the container). The solution can then be further diluted to a working stock solution.
If the product is going to be used for applications requiring absolute asepsis, it's best to filter-sterilize the solution using a sterile and non-pyrogenic 0.2 um membrane before use.
Storage and Stability:
Upon receiving, store the product at -20oC. After reconstitution, store the working aliquots at 2-8oC for no more than 3 months. For extended storage, aliquot the rehydrated solution (≥100 ug/ml) and freeze at -70oC or -20oC. Avoid repeated freezing and thawing. More dilute solutions stored at -20oC will lose activity faster.
Amino Acid Sequence:
MAFPTSQVRR GDFTEDTTPN RPVYTTSQVG GLITHVLWEI VEMRKELCNG NSDCMNNDDA LAENNLKLPE IQRNDGCYQT GYNQEICLLK ISSGLLEYHS YLEYMKNNLK DNKKDKARVL QRDTETLIHI FNQEVKDLHK IVLPTPISNA LLTDKLESQK EWLRTKTIQF ILKSLEEFLK VTLRSTRQT
