$238.80Catalog Number: BX1006-20Size: 20 ug

mIL-6

Recombinant Mouse Interleukin-6 (rmIL-6)

Interleukin-6 (IL-6) is a pro-inflammatory cytokine that also has an important role in immunity. IL-6 induces growth and terminal differentiation of B cells; secretion of immunoglobulins; differentiation and activation of T cells and macrophages; and the induction of acute-phase response proteins. Many types of cells, including macrophages, T cells, fibroblasts, and endothelial cells, produce IL6 in response to stimuli such as bacteria, viruses, and other cytokines, particularly IL-1 and tumor necrosis factor, alpha (TNF). Human IL-6 is active on both mouse and rat cells, while mouse IL-6 has no activity on human cells. Recombinant mouse IL6 is a 22 kDa protein containing 189 amino acid residues.

Other Sizes

BX1006-20:20 ug
BX1006-200:200 ug
BX1006-1000:1 mg

Custom size is also available upon request.

Specifications

Lot Number:On label
Formulation:Lyophilized powder lyophilized from a volatile buffer (50 mM NH4HCO3 , pH 8.0).
Preservative:None
MW:22kD
Purity:>97% on 15% SDS-PAGE
Source:Recombinant mature protein expressed in E. coli (189 amino acid residues).
Sterility:0.2 um membrane-filtered and packaged aseptically.
ED50:ND
Endotoxin:≤0.1 EU/ug protein
QC test:SDS-PAGE

Note: Endotoxin Unit (EU) is determined by Limulus Amebocyte Lysate (LAL) assay.

Reconstitution and Use

Reconstitute the contents of the vial using sterile phosphate-buffered saline (PBS) to a concentration no less than 100 ug/ml and aliquot for future use. (If the initial rehydration is too dilute, activity may be lost due to the non-specific adsorption to the container). The solution can then be further diluted to a working stock solution.
If the product is going to be used for applications requiring absolute asepsis, it's best to filter-sterilize the solution using a sterile and non-pyrogenic 0.2 um membrane before use.

Storage and Stability:

Upon receiving, store the product at -20oC. After reconstitution, store the working aliquots at 2-8oC for no more than 3 months. For extended storage, aliquot the rehydrated solution (≥100 ug/ml) and freeze at -70oC or -20oC. Avoid repeated freezing and thawing. More dilute solutions stored at -20oC will lose activity faster.

Amino Acid Sequence:

MAFPTSQVRR GDFTEDTTPN RPVYTTSQVG GLITHVLWEI VEMRKELCNG NSDCMNNDDA LAENNLKLPE IQRNDGCYQT GYNQEICLLK ISSGLLEYHS YLEYMKNNLK DNKKDKARVL QRDTETLIHI FNQEVKDLHK IVLPTPISNA LLTDKLESQK EWLRTKTIQF ILKSLEEFLK VTLRSTRQT

Quantity

$238.80

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