The sequence of the first five N-terminal amino acids was determined and was found to be, Thr-Leu-Val-Ile-Arg.
The Activity is calculated by the ability to chemoattract Human lymphocytes using a concentration of 0.1-1ng/ml corresponding to a Specific Activity of 1,000,000-10,000,000IU/mg.
The CXCL9 was lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized MIG in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized MIG although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution CXCL9 should be stored at 4oC between 2-7 days and for future use below
-18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Small inducible cytokine B9, CXCL9, Gamma interferon-induced monokine, MIG, chemokine (C-X-C motif) ligand 9, CMK, Humig, SCYB9, crg-10, M119, monokine induced by gamma-interferon.
