Request QuoteCatalog Number: xP524752DNVSize: 0.2-1mg

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Recombinant 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE)

Recombinant 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP524752DNVYeast1mgQuote
EP524752DNVE. coli1mgQuote
BP524752DNVBaculovirus200ugQuote
MP524752DNVMammalian Cell200ugQuote

Protein Information

SpeciesAquifex aeolicus (strain VF5)
UniProt IDO67606
Gene NamemetE; Locus:aq_1710
Protein Name5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Region Expressed1-761
Expression Tag6xHis
Purity>90%
AA SequenceMVKTYAFGFPKLGEKREFKRLLENYWKGKISQEELIKGMEELNLWRANLYRENVEIIPSN ELSYYDFMLDTTVMVGAIPSRFGEYRGLDTYFEMARGKNALELTKWFNTNYHYLVPELEG ENFKLLINRPLEDYKFFKEKGINTVPHVIGLFTFLKLSKAPKRVEEEGLPVIILEKLDTK ERLEKFTKALLPVYKELLESLKNEGVNRVHFEEPALVTDLEDYDWEILNEIYSELSKVGV DLGILTYYDSVSDYDKFVNLPVKALHFDLVSNEENLKNIKEKGFPEDKELIAGIINGRQP WRANLREKVKLLEELLKVSANLVVSNSCPLYHLPVTVEPEDELPEGLKERLSFAKEKLRE IRLIKEALEGKEEALREVESSEKVMTAVFAQNPEVRRRLASLSEKDFQRELPYKERDKIQ RDILKLPLFPTTTIGSFPQTEDVRKMRTAYRTGKISEEEYKNFIKQKIKYAIEVQEDIGL DVLVHGEFERSDMVEYFAIKLDGIATTKHGWVLSYGSRVYRPPIIYGDVSRPAPMTLEEI TYAQSLTNKPVKGMLTGPVTILNWSYYREDVPKREIAYQIALALLDEVKDLENAGIKVIQ IDEPAFREGAPLKKRDWPEYFDWAVKAFRLASRAKPETQIHTHMCYSEFNEIIEYIYQMD FDVISIEASRSKGEIIEVFEKFKGWDRQIGIGVYDIHSPAIPTKEEMKAIMLRAMKVLPK ELLWVNPDCGLKTRRWEEVIPALRNMVDMAKELREEVGVKV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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