Request QuoteCatalog Number: xP528832FHXSize: 0.2-1mg

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Recombinant 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)

Recombinant 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP528832FHXYeast1mgQuote
EP528832FHXE. coli1mgQuote
BP528832FHXBaculovirus200ugQuote
MP528832FHXMammalian Cell200ugQuote

Protein Information

SpeciesPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
UniProt IDO58665
Gene NamepanB; Locus:PH0951
Protein Name3-methyl-2-oxobutanoate hydroxymethyltransferase
Region Expressed1-283
Expression Tag6xHis
Purity>90%
AA SequenceMREITPKRIREMKGKEKITMITAYDYPSALLADKAGFDIVFIGDSLGMVVYGEPSTLNVT MEQMIFHTRAVAKAVKRALVLADMPFGSYEVSVEEGIKNAIKLVQAGADAVKIEGGYDHR KLVKKLVRIGIPVMGHTGLTPQRYLRLGGYRIMGGTEEEVEEILRDAKALEKAGAFAIVL EFVLADVAKLVTEEVSIPTIGIGSGPYVDGQVLVWHDVLGLYESSPPFAKRYANLKEEIL RAISTFREEVKEGKFPGREHYWEFQDKEEFKRIKDNVMKKLNL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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