3-methyl-2-oxobutano-->

Request QuoteCatalog Number: xP501361DUESize: 0.2-1mg

Recombinant 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)

Recombinant 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog Number	Expression Host	Amount	In Stock
YP501361DUE	Yeast	1mg	Quote
EP501361DUE	E. coli	1mg	Quote
BP501361DUE	Baculovirus	200ug	Quote
MP501361DUE	Mammalian Cell	200ug	Quote

Protein Information

Species	Clostridium botulinum (strain 657 / Type Ba4)
UniProt ID	C3L035
Gene Name	panB; Locus:CLJ_B0494
Protein Name	3-methyl-2-oxobutanoate hydroxymethyltransferase
Region Expressed	1-275
Expression Tag	6xHis
Purity	>90%
AA Sequence	MRNTVSTFQELKDRGEKITMLTAYDYSMAKLIDSSGINGILVGDSLGMVCLGYENTLSVT MEDMIHHTKAVVRGTSNALVVGDMPFMSYQTSIYDAVYNAGRFIKEAGAHAVKLEGGATV AEEIKAIVKAQIPVMGHIGLTPQSVNMFGGFKVQGKNEKVAKKLIEDAKILEEAGAFSIV LECIPEKLSKIISESISIPTIGIGAGKYCDGQILVYQDMLSMFSDFKPKFVKSFGNIGES IKDGVSQYIKEVKESKFPEEKHAFKIDDDVINKLY
Storage Buffer	PBS pH 7.4, 50% glycerol
Storage	Store working aliquots at 4^oC for upto a week. Freeze at -20 to -80^oC for long term storage. Avoid repeated freeze/thaw.

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