Request QuoteCatalog Number: xP514366ENUSize: 0.2-1mg

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Recombinant 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)

Recombinant 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP514366ENUYeast1mgQuote
EP514366ENUE. coli1mgQuote
BP514366ENUBaculovirus200ugQuote
MP514366ENUMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain K12 / MC4100 / BW2952)
UniProt IDC4ZRM7
Gene NamepanB; Locus:BWG_0127
Protein Name3-methyl-2-oxobutanoate hydroxymethyltransferase
Region Expressed1-264
Expression Tag6xHis
Purity>90%
AA SequenceMKPTTISLLQKYKQEKKRFATITAYDYSFAKLFADEGLNVMLVGDSLGMTVQGHDSTLPV TVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAATVMRAGANMVKIEGGEWLV ETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAGDQLLSDALALEAAGAQLLVL ECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDAFGITGGHIPKFAKNFLAETGDI RAAVRQYMAEVESGVYPGEEHSFH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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