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Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)

Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP527793DNVYeast1mgQuote
EP527793DNVE. coli1mgQuote
BP527793DNVBaculovirus200ugQuote
MP527793DNVMammalian Cell200ugQuote

Protein Information

SpeciesAquifex aeolicus (strain VF5)
UniProt IDO67089
Gene NameispF; Locus:aq_957
Protein Name2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Region Expressed1-156
Expression Tag6xHis
Purity>90%
AA SequenceMELRIGFGFDSHEFVEGKLLILGGVEIEKDYGLKGHSDGDALLHAITDAILGALGERDIG EIFKDTDPRWKNAPSRIFLEKALEVMSEKGFNISNIDCVIVADRPKIAPHKERIKESLSK LLGIPKERISLKGKRREGFCEGNGLVCMCTVLLVKM
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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