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Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)

Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP493179DJJYeast1mgQuote
EP493179DJJE. coli1mgQuote
BP493179DJJBaculovirus200ugQuote
MP493179DJJMammalian Cell200ugQuote

Protein Information

SpeciesDesulfitobacterium hafniense (strain DCB-2 / DSM 10664)
UniProt IDB8G1T9
Gene NameispF; Locus:Dhaf_0395
Protein Name2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Region Expressed1-158
Expression Tag6xHis
Purity>90%
AA SequenceMLRVGIGYDVHALVAGRPLILAGIDIPHEKGLLGHSDADVLTHTLMDALLGALALGDLGK HFPDTDERYRGISSMKLLEQVMKLLEERGYAIGNIDCIIAAQRPKLAPYIPQMRENLARA LKTDLENVSVKATTTERLGFEGREEGISSQAIVCLVKV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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