Request QuoteCatalog Number: xP481234EOESize: 0.2-1mg

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Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)

Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP481234EOEYeast1mgQuote
EP481234EOEE. coli1mgQuote
BP481234EOEBaculovirus200ugQuote
MP481234EOEMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli O157:H7 (strain EC4115 / EHEC)
UniProt IDB5Z3A9
Gene NameispD; Locus:ECH74115_3998
Protein Name2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Region Expressed1-236
Expression Tag6xHis
Purity>90%
AA SequenceMATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVYALLAHPRVKRVVIAISP GDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWVLVHDAARPCLHQDDLARL LALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGLWHALTPQFFPRELLHDCLTR ALNEGAAITDEASALEYCGFHPQLVEGRADNIKVTRPEDLALAEFYLTRTIHQENT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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