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Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)

Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP455545DSYYeast1mgQuote
EP455545DSYE. coli1mgQuote
BP455545DSYBaculovirus200ugQuote
MP455545DSYMammalian Cell200ugQuote

Protein Information

SpeciesChloroherpeton thalassium (strain ATCC 35110 / GB-78)
UniProt IDB3QV82
Gene NameispF; Locus:Ctha_0565
Protein Name
Region Expressed1-157
Expression Tag6xHis
Purity>90%
AA SequenceMRIGIGIDIHQLVEGRKLIIGGVEIPYEKGLKGHSDADVLLHAISDALLGAAALGDIGKH FPDTDPAYKGIDSQLLLKHVRKLLETHGYKPVNVDSMLLLEKPKIAPHVQKMRENIAACL EVDVDAISVKATTNEKIGYVGRGEGALAHAVCLIEKI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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