Request QuoteCatalog Number: xP480637VFCSize: 0.2-1mg

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Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)

Recombinant 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP480637VFCYeast1mgQuote
EP480637VFCE. coli1mgQuote
BP480637VFCBaculovirus200ugQuote
MP480637VFCMammalian Cell200ugQuote

Protein Information

SpeciesVibrio fischeri (strain MJ11)
UniProt IDB5FAF6
Gene NameispF; Locus:VFMJ11_2178
Protein Name2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Region Expressed1-158
Expression Tag6xHis
Purity>90%
AA SequenceMRIGHGFDVHKFGGEGPVIIGGVSVPYEQGLIAHSDGDVALHALCDALLGAIAEGDIGRH FPDTDDKWKGADSRALLRDVYRRVKEKGFVLGNADVTIIAQAPKMAPHIQAMCNAIAEDL ETELDNVNVKATTSERLGFTGRKEGIACEAVVLLVKAS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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