Direct ELISA, Western Blot, Immuneprecipitation.
MCAF does not bind to plastic as good as most proteins - use higher concentration for proper binding.
1mg/ml in PBS (after reconstitution) Shipping ConditionsAntibody is shipped lyophilized at ambient temperature.
Reconstitute with sterile H2O. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC.
Small inducible cytokine A2, CCL2, Monocyte chemotactic protein 1, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte secretory protein JE, HC11, chemokine (C-C motif) ligand 2, MCP1, SCYA2, GDCF-2, SMC-CF, HSMCR30, MGC9434, GDCF-2 HC11.
In direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories) 1:5,000 dilution will give 0.3 O.D within 10 minutes.
Mouse Anti Human Monoclonal.