The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Asn-Val-Pro-His.
The ED50 of Galectin-7 as determined by hemagglutination test is 0.75~3 ug/ml.
LGALS7 was lyophilized from a concentrated (1mg/ml) solution in 20mM PBS, pH 7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Galectin-7 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized LGALS7 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Galectin-7 should be stored at 4oC between 2-7 days and for future use below -18oC.
Please prevent freeze-thaw cycles.
Galectin-7, Gal-7, HKL-14, PI7, p53-induced gene 1 protein, LGALS7, PIG1, LGALS7B, GAL7, LGALS7A.