Leptin Receptor Chicken Recombinant
Leptin Binding Domain Chicken Recombinant also called Leptin Receptor produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa. Chicken Leptin Receptor consists of the cytokine binding domain of leptin receptor amino acids 420-626 of chicken leptin receptor.
The Leptin Binding Domain is purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-Asp-Val-Asn-Ile
Biological Activity
Biological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.
Formulation
The protein was filter sterilized and stored at 4oC (0.2 to 0.5 mg/ml) solution of Tris-HCl buffer, pH 9.0 with 150mM NaCl.
Physical Appearance
Sterile Filtered colorless solution at a concentration of 0.4 mg/ml.
Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard.
Purity
Greater than 99.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Source
Stability
Sterile solutions at 0.5mg/ml or less are stable at 4oC for several months.
Synonyms
OB Protein, Obesity Protein, OBS, Obesity factor, Leptin Receptor.
