Leptin Human Recombinant, His Tag
Leptin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing amino acids 48-167 and having a total molecular mass of 19 kDa including the 4 kDa His tag.
The Leptin is purified by proprietary chromatographic techniques.
Accession
Applications
1. Positive control for Western blot.
2. Antibody production.
3. Protein assay.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with 1X PBS, 0.1% SDS and 1mM DTT.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.
Source
Stability
Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0oC. Reconstituted Leptin is best stored refrigerated at 4oC.
Please avoid freeze-thaw cycles.
Synonyms
OB Protein, Obesity Protein, OBS, Obesity factor.
