The specific activity was determined by dose dependent ability to inhibit BMP-4 (6.5 ng/mL) induction of alkaline phosphatase production in the MC-3T3-E1 cell line (Mouse chondrogenic cell line) and is typically 10-40ng/ml.
The LEFTY1 was lyophilized from 1mg/ml in 1xPBS.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 90% as obsereved by SDS-PAGE.
It is recommended to reconstitute the lyophilized LEFTY1 in sterile water not less than 100 g/ml, which can then be further diluted to other aqueous solutions.
Lyophilized LEFTY1 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution LEFTY1 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Left-right determination factor 1, Left-right determination factor B, Protein lefty-1, Protein lefty-B, LEFTY1, LEFTB, LEFTYB.
SYF2 Human$148.20
