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Recombinant 3-ketoacyl-CoA thiolase (fadI)

Recombinant 3-ketoacyl-CoA thiolase (fadI) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117KAXYeast1mgQuote
EP001117KAXE. coli1mgQuote
BP001117KAXBaculovirus200ugQuote
MP001117KAXMammalian Cell200ugQuote

Protein Information

SpeciesKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
UniProt IDA6TC20
Gene NamefadI; Locus:KPN78578_26800; ORFs:KPN_02724
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-436
Expression Tag6xHis
Purity>90%
AA SequenceMSQALPLITRQGDRIAIVTGLRTPFARQATAFHGVPAIDLGKMVVGEMLARSDIPAEVIE QLVFGQVVQMPEAPNIAREIVLGTGMSVHTDAYSVSRACATSFQAVANVAESLMAGTIRA GIAGGADSSSVLPIGVSKTLARTLVDANKARTLSQKLKLFSRLRPRDLLPVPPAVAEYST GLRMGDTAEQMAKSWGISREQQDALAHRSHQLAAKAWEEGKLSAEVMTAYAPPFREPLEQ DNNIRKNSTLADYQKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLRPLGY LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTLADLTLIDMHEAFAAQTLANLQCLASD RFAREVLGRSQATGEVDESKFNVLGGSIAYGHPFAATGARMITQTLHEVRRRGGGFGLVT ACAAGGLGAAMIVEAE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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