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Recombinant 3-ketoacyl-CoA thiolase (fadA)

Recombinant 3-ketoacyl-CoA thiolase (fadA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117STSYeast1mgQuote
EP001117STSE. coli1mgQuote
BP001117STSBaculovirus200ugQuote
MP001117STSMammalian Cell200ugQuote

Protein Information

SpeciesShewanella sp. (strain ANA-3)
UniProt IDA0KR49
Gene NamefadA; Locus:Shewana3_0023
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-387
Expression Tag6xHis
Purity>90%
AA SequenceMKQAVIVDCIRTPMGRSKAGVFRNVRAETLSAELMKGLLLRNPQLDPNTIEDVIWGCVQQ TLEQGFNIARNASLLAGIPKTAGAVTVNRLCGSSMEAIHQAARAIMTGMGDTFIIGGVEH MGHVPMNHGVDFHPGLANNVAKASGMMGLTAEMLGKLHGITREQQDAFAVRSHQRAHAAT VEGRFAKEIYGIEGHDANGALIKVLHDEVIRPETSMESLAALRPVFDPANGTVTAGTSSA LSDGASAMLVMEESKARALGLPIRARIRSMAVAGCDAAIMGYGPVPATQKALARAGITVN DLDVIELNEAFAAQSLPCVKDLGLLDVVEDKINLNGGAIALGHPLGCSGARISTTLINLM EHKDATLGLATMCIGLGQGIATVFERV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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