Request QuoteCatalog Number: xP001117BRJSize: 0.2-1mg

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Recombinant 3-ketoacyl-CoA thiolase (fadA)

Recombinant 3-ketoacyl-CoA thiolase (fadA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117BRJYeast1mgQuote
EP001117BRJE. coli1mgQuote
BP001117BRJBaculovirus200ugQuote
MP001117BRJMammalian Cell200ugQuote

Protein Information

SpeciesBacillus subtilis (strain 168)
UniProt IDO32177
Gene NamefadA; aka: yusK; Locus:BSU32830
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-391
Expression Tag6xHis
Purity>90%
AA SequenceMKEAVIVSGARTPVGKAKKGSLATVRPDDLGAICVKETLKRAGGYEGNIDDLIIGCATPE AEQGLNMARNIGALAGLPYTVPAITVNRYCSSGLQSIAYAAEKIMLGAYDTAIAGGAESM SQVPMMGHVTRPNLALAEKAPEYYMSMGHTAEQVAKKYGVSREDQDAFAVRSHQNAAKAL AEGKFKDEIVPVEVTVTEIGEDHKPMEKQFVFSQDEGVRPQTTADILSTLRPAFSVDGTV TAGNSSQTSDGAAAVMLMDREKADALGLAPLVKFRSFAVGGVPPEVMGIGPVEAIPRALK LAGLQLQDIGLFELNEAFASQAIQVIRELGIDEEKVNVNGGAIALGHPLGCTGTKLTLSL IHEMKRRNEQFGVVTMCIGGGMGAAGVFELC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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