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Recombinant 3-ketoacyl-CoA thiolase (fadI)

Recombinant 3-ketoacyl-CoA thiolase (fadI) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117EOPYeast1mgQuote
EP001117EOPE. coli1mgQuote
BP001117EOPBaculovirus200ugQuote
MP001117EOPMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli O81 (strain ED1a)
UniProt IDB7MY17
Gene NamefadI; Locus:ECED1_2805
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-436
Expression Tag6xHis
Purity>90%
AA SequenceMGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARTEIPAEVIE QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSEGKLKEEVMTAFIPPYKQPLVE DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMGDLTLIDMHEAFAAQTLANIQLLGSE RFARDVLGRAHATGEVDESKFNVLGGSIAYGHPFAATGARMITQTLYELRRRGGGFGLVT ACAAGGLGAAMVLEAE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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