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Recombinant 3-ketoacyl-CoA thiolase (fadI)

Recombinant 3-ketoacyl-CoA thiolase (fadI) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117EOBYeast1mgQuote
EP001117EOBE. coli1mgQuote
BP001117EOBBaculovirus200ugQuote
MP001117EOBMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli O127:H6 (strain E2348/69 / EPEC)
UniProt IDB7UFZ9
Gene NamefadI; Locus:E2348C_2481
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-436
Expression Tag6xHis
Purity>90%
AA SequenceMGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAGDLGKMVVGELLARTEIPAEVIE QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSEGKLKEEVMTAFIPPYKQPLVE DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSE RFARDVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT ACAAGGLGAAMVLEAE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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