Request QuoteCatalog Number: xP001117EOESize: 0.2-1mg

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Recombinant 3-ketoacyl-CoA thiolase (fadI)

Recombinant 3-ketoacyl-CoA thiolase (fadI) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117EOEYeast1mgQuote
EP001117EOEE. coli1mgQuote
BP001117EOEBaculovirus200ugQuote
MP001117EOEMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli O157:H7 (strain EC4115 / EHEC)
UniProt IDB5YXY5
Gene NamefadI; Locus:ECH74115_3485
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-436
Expression Tag6xHis
Purity>90%
AA SequenceMGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIE QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVVESLMAGTIRA GIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQLLAE DNNIRGNSSLADFAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMADLTLIDMHEAFAAQTLANIQLLGSE RFAREALGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT ACAAGGLGAAMVLEAE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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