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Recombinant 3-ketoacyl-CoA thiolase (fadI)

Recombinant 3-ketoacyl-CoA thiolase (fadI) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117SYSYeast1mgQuote
EP001117SYSE. coli1mgQuote
BP001117SYSBaculovirus200ugQuote
MP001117SYSMammalian Cell200ugQuote

Protein Information

SpeciesShewanella baltica (strain OS223)
UniProt IDB8EE97
Gene NamefadI; Locus:Sbal223_1597
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-436
Expression Tag6xHis
Purity>90%
AA SequenceMSDRQQVTNAKGERIAIVAGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKLIE QLVYGQVVQMPAAPNIAREIVLGTGMNVSTDAYSVTRACATSFQSAVNVAESIMTGNIEI GIAGGADSSSVLPIGVSKKLAHALVDLNKARSFGQKLQIFRRLGIKDLLPVPPAVAEYST GLSMGQTAEQMAKTYNISRADQDALAHRSHTLASETWASGHLRDEVMVAHVPPYKQFIDR DNNIRENSVLESYAKLRPAFDKQHGTVTAANSTPLTDGASAIILMSEGRAKALGYQPIGY IKSYAFSAIDVWQDMLMGPSYATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFASK KFAEEKLGRNRAIGEIDMSKFNVLGGSLAYGHPFAATGTRLITQVCRELKRRGGGTGLTT ACAAGGLGVAMIVEVE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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