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Recombinant 3-ketoacyl-CoA thiolase (fadA)

Recombinant 3-ketoacyl-CoA thiolase (fadA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117AWNYeast1mgQuote
EP001117AWNE. coli1mgQuote
BP001117AWNBaculovirus200ugQuote
MP001117AWNMammalian Cell200ugQuote

Protein Information

SpeciesAcinetobacter baumannii (strain AB307-0294)
UniProt IDB7H1I1
Gene NamefadA; Locus:ABBFA_003231
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-390
Expression Tag6xHis
Purity>90%
AA SequenceMATLNPRDVVIVDGVRSAMGKSKNGMFRNVRADSLSAELVRALVARNQFDVNEVEDLIWG CVNQTLEQGMNIGRNIGLLAGLPKTVAGQTVNRLCGSSMQAIHTAAAQIATNQGDIFIIG GVEHMGHVGMMHGIDLNPEASKHYAKASNMMGLTAEMLGRMNGITREEQDAFGVESHRRA WAATQEGRFKNEIIGVEGHDANGFKILCDIDEVIRPDANLEAFKALKPVFDPKGGSVTAA TSSALSDGASAMLLMSAERAQALGLKPRAVIRSMAVAGCDAAIMGYGPVPATQKALKRAG LSIADIQTVELNEAFAAQGLSVLKGLGLYDKQDIVNLNGGAIALGHPLGCSGARITTTLL NVMEQQDTQIGLATMCIGLGQGIATVIERV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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