Request QuoteCatalog Number: xP001117EJDSize: 0.2-1mg

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Recombinant 3-ketoacyl-CoA thiolase (fadA)

Recombinant 3-ketoacyl-CoA thiolase (fadA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117EJDYeast1mgQuote
EP001117EJDE. coli1mgQuote
BP001117EJDBaculovirus200ugQuote
MP001117EJDMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli O139:H28 (strain E24377A / ETEC)
UniProt IDA7ZU50
Gene NamefadA; Locus:EcE24377A_4364
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-387
Expression Tag6xHis
Purity>90%
AA SequenceMEQVVIVDAIRTPMSRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQ TLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAARMIMSGDAQACLVGGVEH MGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAAT QSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPAIMGYGPVPASKLALKKAGLSVS DIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLM ERKDVQFGLATMCIGLGQGIATVFERV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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