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Recombinant 3-ketoacyl-CoA thiolase (fadI)

Recombinant 3-ketoacyl-CoA thiolase (fadI) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117SYUYeast1mgQuote
EP001117SYUE. coli1mgQuote
BP001117SYUBaculovirus200ugQuote
MP001117SYUMammalian Cell200ugQuote

Protein Information

SpeciesShewanella halifaxensis (strain HAW-EB4)
UniProt IDB0TL22
Gene NamefadI; Locus:Shal_2671
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-436
Expression Tag6xHis
Purity>90%
AA SequenceMSDRQQVTNARGERIAIVSGLRTPFAKQATAFHGVSALDMGKMVVNELLSRSELNPKEIE QLVYGQVVQMPAAPNIAREIVLGTGMDVSTDAYSVTRACATSFQSTVNVAESIMTGNMDI GIAGGADSSSVLPIGVSKKLAHALVDLNKARSFGQKLAIFRRLGLKDLLPVPPAVAEYST GLSMGQTAEQMAKTYNISRADQDALAHRSHTLATETWNAGKLAEEVMAAHVPPYKSFIDR DNNIRENSTLESYAKLRPAFDRKHGSVTAANSTPLTDGASAVLLMSEGRAKALGYTPIGY IKSYAFTAIDVWQDMLMGPSYATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFASK KFAEEKLGRNRAIGEIDMSKFNVLGGSLAYGHPFAATGTRLVTQVCHELKRRGGGTGLAT ACAAGGLGAAMIVEVE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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