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Recombinant 3-ketoacyl-CoA thiolase (fadI)

Recombinant 3-ketoacyl-CoA thiolase (fadI) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117STEYeast1mgQuote
EP001117STEE. coli1mgQuote
BP001117STEBaculovirus200ugQuote
MP001117STEMammalian Cell200ugQuote

Protein Information

SpeciesSalmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980)
UniProt IDA9MJ36
Gene NamefadI; Locus:SARI_00510
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-436
Expression Tag6xHis
Purity>90%
AA SequenceMRQALPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVSELLARSEIPADAIE QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA GIAGGADSSSVLPIGVSKALARVLVDVNKARTTRQRLTLFSRLRLRDLLPVPPAVAEYST GLRMGDTAEQMAKTYGITREQQDALAYRSHQRAAQAWAEGKLAEEVMTTYAPPYKNPFSE DNNIRGNSTLADYAKLRPAFDRKHGSVTAANSTPLTDGAAAVILMTESRAKELGLRPLGY LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMADLTLFDMHEAFAAQTLANLQLLGSE RFARDVLGRAQATGEVDDTKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT ACAAGGLGAAMVLEAE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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