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Recombinant 3-ketoacyl-CoA thiolase (fadA)

Recombinant 3-ketoacyl-CoA thiolase (fadA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117CXLYeast1mgQuote
EP001117CXLE. coli1mgQuote
BP001117CXLBaculovirus200ugQuote
MP001117CXLMammalian Cell200ugQuote

Protein Information

SpeciesCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
UniProt IDA7MQM5
Gene NamefadA; Locus:ESA_03715
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-387
Expression Tag6xHis
Purity>90%
AA SequenceMEKVVIVDAIRTPMGRSKGGAFRQVRAEDLSAHLMRSLLSRNPQLEASAIDDIYWGCVQQ TLEQGFNIARNAALLAEIPHSVPAVTVNRLCGSSMQALHDAARMIMTGDASVCLVGGVEH MGHVPMNHGVDFHPGLSRNVAKAAGMMGLTAEMLSRMHGISREMQDAFAARSHQRAWAAT QAGHFKQEIIPTSGHDADGVLKRYDFDEVIRPETTAEGLSQLKPAFDPANGTVTAGTSSA LSDGAAAMLVMSESRARELGLTPRARIRSMAVVGCDPSIMGYGPVPASKLALKKAGLTAS DIDLFEMNEAFAAQILPCIKDLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLINLM ERRDAELGLATMCIGLGQGIATVFERV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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