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Recombinant 3-ketoacyl-CoA thiolase (fadA)

Recombinant 3-ketoacyl-CoA thiolase (fadA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117PZMYeast1mgQuote
EP001117PZME. coli1mgQuote
BP001117PZMBaculovirus200ugQuote
MP001117PZMMammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas mendocina (strain ymp)
UniProt IDA4XSM9
Gene NamefadA; Locus:Pmen_1581
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-391
Expression Tag6xHis
Purity>90%
AA SequenceMSLNPRDAVIVDFGRTPMGRSKGGMHRNTRAETMSAHLISKVLERNAKIDPAEVEDVIWG CVNQTLEQGWNIARMASLMTQIPHTSAGQTVSRLCGSSMSALHTAVQAIQTGNGDVFVVG GVEHMGHVGMMHGVDPNPHLSLYAAKASGMMGLTAEMLGKMHGISREAQDAFGERSHRLA HKATVEGKFKDEIIPMQGYDENGFLKVFDYDETIRPETTLESLATLKPAFNPKGGTVTAG TSSQITDGASCMIVMSAQRAQDLGIQPMAVVRAMAVAGVDPAIMGYGPVPSTQKALKRAG LTMADIDFVELNEAFAAQALPVLKDLKLLDKMEEKVNLHGGAIALGHPFGCSGARISGTL LNVMKQNGGTLGVSTMCVGLGQGITTVFERV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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