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Recombinant 3-ketoacyl-CoA thiolase (fadI)

Recombinant 3-ketoacyl-CoA thiolase (fadI) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117AUIYeast1mgQuote
EP001117AUIE. coli1mgQuote
BP001117AUIBaculovirus200ugQuote
MP001117AUIMammalian Cell200ugQuote

Protein Information

SpeciesAeromonas salmonicida (strain A449)
UniProt IDA4SMT9
Gene NamefadI; Locus:ASA_2146
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-436
Expression Tag6xHis
Purity>90%
AA SequenceMKQPLKLTTRQGERIAVVAGLRTPFAKQATAFHGVPAVDLGKLVVSELLARTSLDPKLID QLVFGQVVQMPEAPNIAREIVLGTGMSVTTDAYSVSRACATSFQAVANVTESIMAGTVDI AIAGGADSSSVLPIGVSKKLARALVDLNKARNLQQRFNILRALRLKDLMPVPPAVAEYST GLSMGQTAEQMAKSHQISREAQDALAHRSHTLAAQAWADGKLSGEVFTAHVPPYKSPLER DNNIRESSDLASYAKLKPVFDRVHGTVTAANATPLTDGAAAVLLMREGRAKELGLEPLGY IRSFAFSAIDVWQDMLMGPSYATPLALERAGITLADLTLIDMHEAFAAQTLANLKMFASA EFARDKLGRSQAIGEVDMDKFNVLGGSLAYGHPFAATGARMITQTLHELRRRGGGLGLNT ACAAGGLGVAMVLEVE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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