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Recombinant 3-ketoacyl-CoA thiolase (fadA)

Recombinant 3-ketoacyl-CoA thiolase (fadA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001117EIZYeast1mgQuote
EP001117EIZE. coli1mgQuote
BP001117EIZBaculovirus200ugQuote
MP001117EIZMammalian Cell200ugQuote

Protein Information

SpeciesEnterobacter sp. (strain 638)
UniProt IDA4WFX5
Gene NamefadA; Locus:Ent638_3950
Protein Name3-ketoacyl-CoA thiolase
Region Expressed1-387
Expression Tag6xHis
Purity>90%
AA SequenceMEKVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPALEASAIDDIYWGCVQQ TLEQGFNIARNASLLAEIPHSVPAVTVNRLCGSSMQALHDAARMIMTGDAQVCMVGGVEH MGHVPMSHGVDFHPGMSRNVAKAAGMMGLTAEMLSRMHGISREMQDQFAARSHARAWAAT QSGAFKNEILPTGGHDVDGVLKQYYYDEVIRPETTVEALSALRPAFDPVTGTVTAGTSSA LSDGAAAMLVMSESRARELGLTPRARIRSMAVVGCDPSIMGYGPVPASKLALKKAGLTAS EIDVFEMNEAFAAQILPCIKDLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLINLM ERKDAQFGLATMCIGLGQGIATVFERV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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