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Recombinant 3-keto-steroid reductase (erg27)

Recombinant 3-keto-steroid reductase (erg27) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP010776SXVYeast1mgQuote
EP010776SXVE. coli1mgQuote
BP010776SXVBaculovirus200ugQuote
MP010776SXVMammalian Cell200ugQuote

Protein Information

SpeciesSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
UniProt IDO74732
Gene Nameerg27; ORFs:SPBC1709.07
Protein Name3-keto-steroid reductase
Region Expressed1-338
Expression Tag6xHis
Purity>90%
AA SequenceMSFRKYALITGSNSGLGFGIATRLLQFYQPRLQDEPEVFTVILTCRSREKAEDACRRLKE FFPDRKIRLEYVLLDLSNMASVEAAVQDIATRFPKLDFVYLNAGAWDLEGIQWLKAIFST LINPIQALTHPTFYKETAGRVSNDSLGYIFESNVFGHFYLKNRLAELKVLRSSTKVVLTS SLVAEKKSLDFEDLQCFHGEQPYQSSKRLLDVLHYAELEKGLPFEQYLVHPGLCTTNMYE TFLGPILVMCAKLGFYICRLLGSPWHTISPYVAAFAFLWTALHATKEDQSIKWGAAVTRF GHERVLSTPVELILPSEQEKALEYMTKLYQEWKKKLVS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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