Request QuoteCatalog Number: xP673668FFZSize: 0.2-1mg

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Recombinant 2-keto-4-pentenoate hydratase 1 (mhpD1)

Recombinant 2-keto-4-pentenoate hydratase 1 (mhpD1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP673668FFZYeast1mgQuote
EP673668FFZE. coli1mgQuote
BP673668FFZBaculovirus200ugQuote
MP673668FFZMammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas putida (Arthrobacter siderocapsulatus)
UniProt IDQ400K2
Gene NamemhpD1; aka: orcD
Protein Name2-keto-4-pentenoate hydratase 1
Region Expressed1-265
Expression Tag6xHis
Purity>90%
AA SequenceMTISQETLERLAADLRRAEQQGEAIPPLRDAIGAENGEAAYAIQRLNVAHALAAGRRLVG RKVGLTNPKVQAQLGVDQPDFGCLFADMAYGDNETVPFDRVLQPKIEREIALVLERDLPA RTPTWPNVANRHRLGACRPLEIVGTVSPTWNIRFADTVADNASSGLFVLGGPARRLDGLD LRGAAMRMTRNDELVSSGSGAECLGHPLNAAVWLARTLARLGDPLKAGDIVLTGALGPMV AVNAGDRFDAEIDGLGRVGVTFSQS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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