Request QuoteCatalog Number: xP303543ENVSize: 0.2-1mg

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Recombinant 2-keto-4-pentenoate hydratase (mhpD)

Recombinant 2-keto-4-pentenoate hydratase (mhpD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP303543ENVYeast1mgQuote
EP303543ENVE. coli1mgQuote
BP303543ENVBaculovirus200ugQuote
MP303543ENVMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain K12)
UniProt IDP77608
Gene NamemhpD; Locus:b0350, JW0341
Protein Name2-keto-4-pentenoate hydratase
Region Expressed1-269
Expression Tag6xHis
Purity>90%
AA SequenceMTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHINVQHDVAQGRRVVGRK VGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQPRIEAEIALVLNRDLPATD ITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNASCGVYVIGGPAQRPAGLDLKN CAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASLGEPLRTGDIILTGALGPMVAVN AGDRFEAHIEGIGSVAATFSSAAPKGSLS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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