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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP389162STOYeast1mgQuote
EP389162STOE. coli1mgQuote
BP389162STOBaculovirus200ugQuote
MP389162STOMammalian Cell200ugQuote

Protein Information

SpeciesShewanella loihica (strain ATCC BAA-1088 / PV-4)
UniProt IDA3QIP0
Gene NameleuA; Locus:Shew_3472
Protein Name2-isopropylmalate synthase
Region Expressed1-523
Expression Tag6xHis
Purity>90%
AA SequenceMSNRVIIFDTTLRDGEQALAASLTVKEKLQIALSLERLGVDVMEVGFPVSSPGDFESVQT IARTIKNSRVCALARALEKDIDAAAQSLSVAEQFRIHTFISTSTIHVESKLKRSFDQVLD MAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEAAIKAGAKTINIPDTVGYTVPSEF GGIIQNLFDRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTVNGIGERAGNCS LEEIAMIIATRKASLGLETGINAKEIHRTSNLVSQLCNMPVQANKAIVGANAFTHSSGIH QDGVLKSQNTYEIMTPESIGLNRNNLNMTSRSGRHVIKHRMEEMGYGEQDYDMDTLYEAF LKLADKKGQVFDYDLEALVYVEAQAEDENHYGLQQLVVHSDSTQGQATATVTLSIDGKAV TEAATGNGPVDAAYKAIARASGCEINISSYQLSAKGQGQDALGQVDITAKYHDQSFHGVG LATDVVEASAAALIHVMNLTWRADKVAENKHKLKQARTELGGV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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