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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP374648YAKYeast1mgQuote
EP374648YAKE. coli1mgQuote
BP374648YAKBaculovirus200ugQuote
MP374648YAKMammalian Cell200ugQuote

Protein Information

SpeciesYersinia enterocolitica serotype O:8 / biotype 1B (strain 8081)
UniProt IDA1JJH7
Gene NameleuA; Locus:YE0654
Protein Name2-isopropylmalate synthase
Region Expressed1-520
Expression Tag6xHis
Purity>90%
AA SequenceMSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVRT IAQQMKNSRVCALARCVDKDIDVAAEALRIAEAFRIHVFLATSTLHIESKLKRSFDDVLA MAVHSVKRARNYTDDVEFSCEDAGRTPIDNLCRIVEAAINAGATTINIPDTVGYTTPYQF GGIITDLYQRVPNIDKAIISVHCHDDLGMSVANSITAVQAGARQVEGTINGLGERAGNCS LEEVIMAIKVRENMLGVHTNINHQEIYRTSQLVSKLCNMPIPANKAIVGSNAFAHSSGIH QDGVLKNRENYEIMTPQSIGLKEVQLNLTSRSGRAAVKHRMEEMGYQDKDYNLDSLYDAF LKLADKKGQVFDYDLEALAFINKQQEEPEHYRLDYFSVQSGSSVMATASVKLVCGEEIKS EAATGNGPVDAVYQAINRITDYPIELVKYQLSANGQGKDALGQVDIVVDHKGRRFHGVGL ATDIVESSAKALVHVLNNIWRAHQVEIEKQRLQQNNQEMV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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