Request QuoteCatalog Number: xP388155PZESize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP388155PZEYeast1mgQuote
EP388155PZEE. coli1mgQuote
BP388155PZEBaculovirus200ugQuote
MP388155PZEMammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain MIT 9301)
UniProt IDA3PDH0
Gene NameleuA; Locus:P9301_11721
Protein Name2-isopropylmalate synthase
Region Expressed1-546
Expression Tag6xHis
Purity>90%
AA SequenceMSKNPGRILIFDTTLRDGEQSPGASLNLEEKLAIAHQLARLGVDVIEAGFPFASPGDFKA VNKIASAVGKEHGPTICGLARASKGDIKACYEAVSPAPKKRIHTFIATSDIHLKHKLKKS RKDVLQIVPEMVNYAKSLVDDIEFSCEDASRSDPDFLYEVIQLAISAGATTINIPDTVGF TTPSEFGNLIADINKNVPNIDEAVISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE RAGNASLEELVMALHVRKSFFNSFFKRNPDSPTPLTAIRTEEITKTSRLVSNLTGMTVQP NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEE MGYDLSREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQLPEAKFQLSLVQVSCGNAS KPTATISLLNTEDNTEDTAVSIGTGPVDAVCEALNKLAKVPNELIEFSVKSVTEGIDALG EVTIRIRRDNKIYSGHSADTDVVVAAANAYINALNRLVFSEKKNSIHPQFDNLENSNNTY ISNPAN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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