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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP380920AUCYeast1mgQuote
EP380920AUCE. coli1mgQuote
BP380920AUCBaculovirus200ugQuote
MP380920AUCMammalian Cell200ugQuote

Protein Information

SpeciesAcidovorax sp. (strain JS42)
UniProt IDA1W6T7
Gene NameleuA; Locus:Ajs_1774
Protein Name2-isopropylmalate synthase
Region Expressed1-512
Expression Tag6xHis
Purity>90%
AA SequenceMTDKLIIFDTTLRDGEQSPGASMTRDEKLRIARQLERLRVDVIEAGFAASSNGDFESVQA IARAVKDSTVCSLARANDRDIARAAEALKEANRARIHTFIATSALHMEKKLRMTPEQVLE QARQAVRFARNLVEDIEFSPEDGYRSDPDFLCRVIETVIAEGATTINVPDTVGYAIPELY GNFIKNLRERIPNSDKAIWSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNC SLEEVVMAVKTRRDYFGLDVGIDTQHIVAASRMVSQTTGFVVQPNKAVVGANAFAHASGI HQDGVLKARDTYEIMRAEDVGWSANKIVLGKLSGRNAFKQRLQELGVQLESEGEINVAFA KFKELADRKSEIFDEDILALVSDESVTSEKEQFGFVSLAQHSETGERPEATIVFTVDGKE VRSSSEGNGPVDASMKAIEAHVRSGAEMVLYSVNAISGSTESQGEVTVRLQSAGRVVNGV GADPDIVVASAKAYLSALNKLQSKAERVAAQG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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