Request QuoteCatalog Number: xP384013DUTSize: 0.2-1mg

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Recombinant 2-isopropylmalate synthase (leuA)

Recombinant 2-isopropylmalate synthase (leuA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP384013DUTYeast1mgQuote
EP384013DUTE. coli1mgQuote
BP384013DUTBaculovirus200ugQuote
MP384013DUTMammalian Cell200ugQuote

Protein Information

SpeciesClostridium thermocellum (strain ATCC 27405 / DSM 1237)
UniProt IDA3DF94
Gene NameleuA; Locus:Cthe_1391
Protein Name2-isopropylmalate synthase
Region Expressed1-511
Expression Tag6xHis
Purity>90%
AA SequenceMRRIRIFDTTLRDGEQTPGVNLNIQEKVDIAKQLARLGVDVIEPGFPLTSPGDFEAVQRI AREVEGPYICGFSRAIIRDIDETWKAIKDAQKKCFHIFISSSDIQIKHQLGKTEKDVLEI VKSTVYHAKQYTDEVEYSPMDASRTRLEFLYEVIEAAIDNGATVINIPDTVGYATPIEFG ELIQKIRKNVRNIDKAIISVHCHNDLGMAVANSIVAAMNGAQQIECTINGVGERAGNAAL EEVVTHIAARKDYLGFETGIDLSQLYKTSKIVSRYMGIPIPVNKPIVGKNVFTHESGIHQ DGVLKERSTYEVIDPRLVGRDDSVILLGKHSGRHALKVEAEKLGYDLDEERLNKLFNDFK KLTDVKKNVTTADLESLIIESAAKAVEEAYVLEKIRVVSGNIETPSAKVVIKDSKGNLLE AEQTGNGPVDAVFKAINSVIKETENLTLYKYSVSAVTEEMESLGEVSVTLREKEKLYTGI GTHTDIITSSAIAYIDAINKAIAANARAQKN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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